A study on the use of spectrophotometry

Abstract Background This study aimed to quantify the active biological compounds in C. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenylpicrylhydrazyl DPPH as well as the total antioxidant potential, using the ferric reducing power FRAP assay. Results Spectrophotometric assays in the ultraviolet-visible UV-VIS region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography HPLC was used to identify and quantify phenolic compounds depending on the method of extraction.

A study on the use of spectrophotometry

Simplified energy-level diagram of the frontier orbital of a porphyrin in free-base state H2P, in a regular and in a SAT metalloporphyrin. While the natural porphyrin derivatives are exclusively hydrophobic, some artificial porphyrins having ionic substituents made it possible to prepare water-soluble metalloporphyrins of both regular and SAT type.

Kinetically labile complexes are mostly examined in the excess of the ligand. In the case of metalloporphyrins, however, metal ions are applied generally in excess, especially for spectrophotometric measurements, partly because of the extremely high molar absorbances mainly at the Soret-bands of the porphyrins.


The formation of kinetically labile SAT complexes, deviating from the regular metalloporphyrins, is an equilibrium process. It can be spectrophotometrically monitored because the absorption and emission bands assigned to ligand-centered electron transitions undergo significant shift and intensity change upon coordination of metal ions.

Suggest Documents Overview[ edit ] Spectrophotometry is a tool that hinges on the quantitative analysis of molecules depending on how much light is absorbed by colored compounds. Spectrophotometry uses photometersknown as spectrophotometers, that can measure a light beam's intensity as a function of its color wavelength.
Spectrophotometry - Wikipedia Thus, their absorption bands significantly overlap with the emission spectrum of the solar radiation reaching the biosphere, resulting in efficient tools for conversion of radiation to chemical energy. In such a conversion, the favourable emission and energy transfer properties of porphyrin derivatives are indispensable as in the case of chlorophylls, which contain magnesium ion in the core of the macrocycle.
Absorption spectrophotometry vs. Ultraviolet-Visible Range Spectrophotometry Contact Us Absorption spectrophotometry vs. Ultraviolet-Visible Range Spectrophotometry Spectrophotometry is a technique employed when quantitative measurement and analysis of specific spectra of light is required.

Manganese—porphyrin complexes have more extensively studied because were found to be similar to the biologically active compounds Nakanishi et al.

The very important properties that influence the reactivity of the Mn III -porphyrin concerns the changes in the oxidation states of Mn in the complexes for its high reactivity with O2 Cuzzocrea et al.

Such very slow rates have been discussed in terms of the rigidity of the planar porphyrin framework. The electronic nature of porphyrins, and also the steric accessibility of the bound metal center, www.

A Wavelength nm Fig. The investigation of the kinetics of the complexation of Nsubstituted porphyrins with several metal ions, showing that N-alkylporphyrins form metal complexes much faster than corresponding non-N-alkylated porphyrins Aizawa et al.

The formation of metalloporphyrins can be accelerated substantially by the use of an auxiliary complexing agent.

The Use of Spectrophotometry UV-Vis for the Study of - InTechOpen - rutadeltambor.com

Molecular aggregates of several dyes have been studied as organic photoconductors Borsenberger et al. Some properties of molecular structure of aggregates permit their use in superconductivity, and other processes Kobayashi,; Schouten et al.

Aggregation of small organic molecules to form large clusters is of large interest in chemistry, physics and biology. In nature, particularly in living systems, self-association of molecules plays a very important role; an example is given by molecular aggregates of chlorophyll that have been found to mediate the primary light harvesting and charge-transfer processes in photosynthetic complexes Creightonet al.

In fact, light-harvesting www. Self-assembly of molecules, driven by not-covalent intermolecular interactions, is a convenient route for manufacturing of new functional materials Lidzey at al.

Recently, porphyrin assembly has been used for light-driven energy transduction systems, copying the photophysical processes of photosynthetic organisms Choi et al.

A study on the use of spectrophotometry

The aggregation of porphyrins, changing their spectral and energetic characteristics, influences their efficacy in several applications thus, it is very important take on detailed informations about the formation dynamic and on the typology of aggregates.

In the metal complexation of porphyrins the efficiency reaction is affected by their aggregation Yusmanov et al, Several authors have observed that in the photogeneration of H2O2 by porphyrins, the efficiency of production was highly dependent on their aggregation state Komagoe et al, The diverse chemical and photophysical properties of porphyrins are in many cases due to their different aggregation mode and, as a result of interchromophoric interactions, perturbations in the electronic absorption spectra of dyes occur.

Because the aggregates of porphyrins show peculiar spectroscopic properties, the molecular associations of porphyrins were generally investigated using UV—vis absorption and fluorescence spectroscopy Ohmo, The J-type aggregates side-by-side were formed for transitions polarized parallel to the long axis of the aggregate, while H-type face-to-face for transitions polarized perpendicular to it Fig.

J-aggregates are formed with the monomeric molecules arranged in one dimension such that the transition moment of the monomers are parallel and the angle between the transition moment and the line joining the molecular centers is zero Bohn, The strong coupling of monomers results in a coherent excitation with a red-shift relative to the monomer band.

H-aggregates are again a one-dimensional arrangement of strongly coupled monomers, but the transition moments of the monomers are perpendicular ideal case to the line of centers. On the contrary of J-aggregates, the arrangement in H-aggregates is face-to-face. The dipolar coupling between monomers leads to a blue shift of the absorption band Czikklely et al.

How to Do Spectrophotometric Analysis: 13 Steps (with Pictures)

The H-aggregates are not known to have sharp spectra like the Jaggregates; nevertheless, there are many examples where the spectroscopic blue shift, evident for formation of H-aggregates, was observed. Schematic representation of H and J aggregates.

These aggregated are of particular interest because the highly ordered molecular arrangement present unique electronic and spectroscopic properties that can be predicted Fidder et al.

Due to the distinct optical properties, also the control of the formation of H- and J-aggregated states of dyes has attracted much research interest Maiti et al. As a result, a wide variety of self-assembled porphyrin structures are highly desirable for practical use, which can be applied to nonlinear optical materials Collini et al.

In general, the aggregate formation of porphyrins have been studied in solution, and their physicochemical properties can be affected by the ionic strength, nature of the titrating acid, www.

H- and J-aggregates was formed by simply mixing aqueous solutions of two kinds of porphyrins with opposite charges Xiangqing et al. Moreover, self-assembly can be mediated by templates that allows for obtaining aggregates with additional properties, e.

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In these systems, coupling of strong transition dipoles can result in a perturbations to the electronic absorption spectra of monomer with hyposochromic and bathochromic shift of the monomer Soret band, for H and J aggregates formation respectively Gourterman et al.6 The Use of Spectrophotometry UV-Vis for the Study of Porphyrins Rita Giovannetti University of Camerino, Chemistry Section of School of Environmental Sciences, Camerino Italy 1.

In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light, near-ultraviolet, and near-infrared, but does not cover time-resolved .

Our authors and editors. We are a community of more than , authors and editors from 3, institutions spanning countries, including Nobel Prize winners and some of . Spectrophotometry is the quantifiable study of interaction of electromagnetic radiations with the matter. Electromagnetic radiations do not require any medium for its transmission.

It consists of two components, electric and magnetic field. Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is used in many areas of science including microbiology, biochemistry, forensics, physics, and medical health.

You can use it to measure certain ingredients in a drug to make sure it is effective and safe for consumers. In this lesson we will learn about Beer's Law and how to use spectrophotometry to determine either molar absorbance or concentration in the Beer's Law equation.

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